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2 1 0 0(B) . 14 and 2 M NaCl prior to fixation in osmium tetroxide and embedding. The chromatin is extracted and the RNP network apparently corresponds to the various filaments and granules of the interchromatin areas (I) to which the nucleolus (No) seems to be attached. ~22,000. From Busch and Smetana (79). 1. 18. “Nucleoli” isolated by the procedure of Penman (81). 1. ISOLATION OF NUCLEI AND NUCLEOLI 41 digested with DNase. The nuclear lysate was sedimented and washed with HSB buffer twice and processed for electron microscopy.
Phase micrograph of Novikoff hepatoma nuclei, 4 minutes after the addition of detergent to whole-cell suspension. Nucleoli are inconspicuous due to chromatin clumping (pointers). The apparent gaps along nuclear perimeters (arrows) are a refractile artifact of phase microscopy. x 1300. From Daskal ef al. (58). 9. Electron micrograph of the nuclear preparation from Fig. 8. Condensed chromatin (CCh) around nuclear peripheries gives the reticulated appearance of nuclei in light microscopy (Fig. 8).